PCR 2X PREMIX FAILSAFE 20KB LONG 2.5MLTwelve 2X PreMixes, individual components of the FailSafe PreMix Selection Kit for PCR optimization, and the bundled post optimization FailSafe PCR System. These are 2. 5 mL individual components of the FailSafe PCR Premix Selection Kit and FailSafe PCR System. FailSafe PCR 2X PCR PreMixes contain 100 mM Tris HCl (pH 8. 3), 100 mM KCl, 400 M of each dNTP, and varying concentrations of both the MgCl2 (3 7 mM) and FailSafe PCR Enhancer (0 8X). Before