6X Tris-Glycine SDS Sample buffer, pH 6.8, 50mL" Specification Value Concentration 6x Name Tris Glycine SDS Sample buffer pH at 1x 6. 8 Volume 50mL SKU TGS 06 0005 Composition at 6x Tris. HCl 375 mM, SDS 9%, glycerol 50%, bromophenol blue (Non reduced) 0. 015% . Add mercaptoethanol (9% V V) before use (Reduced). A denaturing sample loading buffer containing SDS and reducing agents for preparing protein samples for SDS PAGE analysis. This formulation ensures complete protein denaturation and